Von Kossa kit is used for calcium deposits and calcium salt visualization. Silver ions from silver nitrate replace carbonate and phosphate calcium ions that, under strong source of light, in turn create microscopically visible silver glow.
Treating the preparation with lithium carbonate prevents false positive staining from occurring.
Counterstaining is achieved with Nuclear Fast Red (Kernechtrot) reagent.
Resources
1 Kit - 100 Test
