- Electron Microscopy
EA 65 reagent Pap 3D - Papanicolau
Polychromatic reagent for staining and differentiation of squamous cells. Counterstain for non-gynecology samples in cytology, stains the cytoplasm light blue-green and pink.
EA 65 reagent, Pap 3D reagent is an alcoholic solution of two acid dyes, Eosin Y and Light Green SF, with added phosphotungstic acid (PTA).
The first step in using the Papanicolaou staining method implies nuclear staining with a hematoxylin solution, and next two steps consist of counterstaining using the monochromatic OG-6 or Orange II reagents and one of the polychromatic EA reagent formulations.