Five-reagent kit for staining nerve cells
Bielschowsky kit is intended for visualizing nerve cells in histology (dendrites and axons) and in neuropathology for diagnosing pathology processes, such as Alzheimer's disease that creates distinctive structures known as senile plaques and neurofibrillary tangles. Silver impregnation method acc. to Bielschowsky enables nervous system' s neurons and axons staining; first through impregnation in silver nitrate solution, then through secondary impregnation in silver-ammonia solution, and finally through silver ion reduction - elementary silver precipitation on the section using developer containing formaldehyde. Sodium thiosulfate solution is used for rinsing and removing non-bound silver.
Result
Nerve endings, neurofibrils, neurofibrillary tangles, neural plaques, nuclei - dark brown to black
Background - yellow to light brown
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