Products

DOTAP Methyl Sulfate
Code : 14475MS-100
RUO

1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) methyl sulfate

Features:

  • Non-cytotoxic at working concentrations
  • High efficiency and reproducibility
  • Cost effective and easy to use

DOTAP MS (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate) is a proven reagent for the highly efficient liposomal transfection of DNA, RNA, oligonucleotides, and some other negatively charged large biomolecules. Several other techniques of introducing genetic material into cells may suffer from inadequate reproducibility, cytotoxicity, inconvenience of process, and low transfection efficiency. DOTAP avoids these drawbacks and works by encapsulating the negatively charged DNA within a spontaneously formed liposomal vesicle. This DOTAP/DNA complex can then fuse with the cell membrane and insert DNA directly into the cytoplasm. This mechanism of action ensures transfection occurs efficiently and with little to no cytotoxicity.

Contact us to know about our cGMP grade DOTAP Methyl Sulfate manufactured under 21 CFR part 210,211.

APPLICATION(S):

Transfection of mammalian cells with DNA, RNA, and oligonucleotides

SHIPPING REQUIREMENTS:

Dry Ice

100 mg
Documentation will be ready soon, in the meanwhile please feel free to contact us at specialist@histoline.com