Bead- and column-based separation methods rely heavily on the speed and ease of affinity binding systems. Ligands such as streptavidin, antibodies and lectins are used both to capture specifically-tagged targets and for the isolation of cells and biomolecules that naturally express the ligand binding partner. The unique saccharide-binding properties of plant lectins, such as Concanavalin A (Con A) have made them useful for the labeling and isolation of glycan-presenting cells and glycoproteins in serum and cell lysate. Lectins have additionally been used in cell adhesion studies, to effect lymphocyte activation, and to explore carbohydrate-based therapeutics.
10 ml
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