The probe is a double-stranded DNA probe cocktail containing TERC probe, 332 kb in size, labeled with red fluorescence dye (excitation 589 nm and emission 615 nm, similar to Texas Red), and chromosome 3 centromere probe labeled with green fluorescence dye (excitation 496 nm and emission 520 nm similar to FITC). The probe cocktail detects human TERC gene at chromosome 3q26 region and chromosome 3 alpha-satellites DNA D3Z1 in standard cytogenetic preparation, cytology preparation, or formalin-fixed paraffin embedded (FFPE) tissue section by fluorescence in situ hybridization (FISH) methodology.
The probe has been demonstrated to bind specifically to the chromosome 3 region 3q26 (Red) and centromere of chromosome 3 (Green) by metaphase FISH in normal lymphocytes. This probe can detect multiple copies in human cancer cell lines which are known to have gain of chromosome 3.
