Hematoxylin acc. to Weigert is used mostly in combination with various special (trichrome) stains. Because of its resistance to acid solutions, it retains the dye and stains nuclear membranes. Unlike the standard hematoxylins used in histology, such as hematoxylins acc. to Harris, Mayer, Mayer-Lillie, and Gill, Hematoxylin acc. to Weigert contains ferric ions that create resistance to acid solutions and sudden pH val ue changes
Result
Nuclei - blue-purple (if the slide is stained only with Hematoxylin acc. to Weigert). Combined with special staining kits, nuclei turn blue-black
100 mL