- Microscopia electronica
The oligonucleotide probe detects Lambda light chain mRNA in formalin-fixed, paraffin-embedded human tissues by in situ hybridization.
The light chains of immunoglobulin molecules have two antigenic types: kappa and lambda. A given immunoglobulin molecule contains two identical light chains, either kappa or lambda. Therefore, the clonal nature of any immunoglobulin producing cell population can be determined by the light chain structure of the immunoglobulin that the cell produces. Lambda light chain restriction and its mRNA levels can be
determined using in situ hybridization techniques. A mixture of non specific oligonucleotides that are of similar length to that of the Lambda probe is provided as the negative control.