Chromogenic In Situ Hybridization (CISH) allows detection of gene amplification, chromosome translocations and chromosome number using conventional enzymatic reactions under the brightfield microscope on formalin-fixed, paraffin-embedded (FFPE) tissues.
CISH is a practical, cost-effective, and valid alternative to fluorescent in situ hybridization (FISH) and can be easily integrated into routine testing in laboratories.
Compared to FISH chromogenic in situ hybridization offers three important advantages:
1. the histological details of the paraffin section are generally better appreciated with a bright field
2. the morphological details are readily apparent using low-power objectives
3. the probe signals are not subject to rapid fading
(Chang et al. HER-2/neu oncogene amplification by chromogenic in situ hybridization in 130 breast cancers using tissue microarray and clinical follow-up studies. J Korean Med Sci 2004; 19: 390-396)

Safe and simple detection of genomic alterations using light microscopy! SPoT-Light® CISH™ Probes and CISH™ Detection

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